Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough

Table 1 Genome editing efficiency for the ADE2 deletion

The genome-editing efficiency of the ADE2 deleted strains were analyzed, including different recovery periods after electroporation and different concentration of plasmid for electroporation. For different recovery times, the concentration of plasmid DNA was 250 ng. For different plasmid concentrations, the recovery time was 48 h. The concentration of DNA template donors was 1 µg. The values shown represent at least three independent experiments (data are mean ± SD). The significant difference in 24 and 48 h recovery time from 5 h recovery time and in 500 ng plasmid transformed from 250 ng plasmid transformed was confirmed by Student’s t-test (*p < 0.05, **p < 0.01)

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