Fig. 3
From: Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough
Agarose gel electrophoresis of colony PCR analysis for the genome-edited strains. PCR amplified the DNA contained A the NTH1 gene by nth1CK1/nth1CK2 primer set and B the HSP12 gene by hsp12CK1/ hsp12CK2 primer set. Lane 1, DNA ladders, lane 2, wild-type strain, lanes 3–4, NTH1 deleted strain, lanes 5–6, HSP12 deleted strain, lanes 7–10, NTH1/HSP12 double deleted strain. PCR analysis was employed from two (Δnth1 and Δhsp12) or four (Δnth1/Δhsp12) separate colonies of edited yeast strains