Fig. 1
From: Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough
The homologous recombination DNA templates for ADE2 in CRISPR-Cpf1 system. A Scheme showing the design of the homologous recombination DNA templates for ADE2 gene and the genome-editing efficiency using CRISPR-Cpf1 in S. cerevisiae system. Data were derived from three separate experiments and are presented as the means ± SD. B–D Colonies of the strains with ADE2 gene disruption were shown in red color. The homologous recombination DNA templates used for CRISPR editing were B 1 kb dsDNA, C 120 bp dsDNA, and D 120 bp ssDNA