Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol

Table 1 Fermentation characteristics of C. glutamicum strains cultivated in CGXIIP medium supplemented with initial 40 g/L glucose measured at 24 h

Strain	Biomass (OD600)	Consumed glucose (g/L)	Acetoin (g/L)	2,3-Butanediol (g/L)	2,3-Butanediol Yield (g/g glucose)	Acetoin + 2,3-Butanediol Yield (g/g glucose)
CGK1	26.30 ± 0.54	36.30 ± 0.45	3.50 ± 0.18	12.38 ± 0.21	0.341 ± 0.008	0.430 ± 0.005
CGK2	24.42 ± 0.52	40.95 ± 0.09	2.43 ± 0.02	16.58 ± 0.13	0.405 ± 0.004	0.455 ± 0.010
CGK3	22.45 ± 0.25	38.26 ± 0.28	1.04 ± 0.03	16.47 ± 0.31	0.430 ± 0.010	0.448 ± 0.008
CGK4	24.68 ± 0.83	42.28 ± 0.31	1.20 ± 0.03	18.27 ± 0.26	0.432 ± 0.010	0.451 ± 0.012
CGK5	20.08 ± 1.35	33.30 ± 1.26	0.84 ± 0.04	14.40 ± 0.57	0.432 ± 0.011	0.448 ± 0.013

Acetoin + 2,3-Butanediol yield was calculated by converting 2,3-butanediol to acetoin and adding the content of acetoin
Data are average values and standard deviations of triplicate experiments

ISSN: 1475-2859