Table 1 The bacterial strain, plasmids, primers and gene used in this study
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
Strain, plasmid, primer or gene | Relevant features or sequence | References |
|---|---|---|
Strain | ||
L. lactis subsp. cremoris NZ9000 | MG1363 nisRK ΔpepN | NIZO |
Plasmids | ||
pNZ8148 | pSH71 derivative, PnisA, Cmr, nisin-controlled expression | |
pSDBA3b | pNZ8148 containing gene fusion of sp Usp45, b-domain and acmA3b | [31] |
pSD-EVA | pNZ8148 containing gene fusion of spUsp45, eva and acmA3b | [39] |
pSD-EVA-flag | pNZ8148 containing gene fusion of spUsp45, flagtag, eva and acmA3b | This study |
pSD-ZHER-flag | pNZ8148 containing gene fusion of spUsp45, flagtag, zher and acmA3b | [38] |
pJET-ZIL | pJET containing a fusion gene of ZIL, tolA protein, and AviTag consensus | This study |
pSD-ZIL | pNZ8148 containing gene fusion of spUsp45, zil and acmA3b | This study |
pSD-ZIL-flag | pNZ8148 containing gene fusion of spUsp45, zil-flag and acmA3b | This study |
Primers | ||
ZIL_F_BamHI | ATTAGGATCCGTTGACGCTAAATATGCTAAAG | This study |
ZIL_R_EcoRI | ATTTGAATTCTTTTGGGGCTTGACTATC | This study |
Usp1-NcoI | ATAACCATGGCTAAAAAAAAGATTATCTCAGCTATTTTAATG | [31] |
FLAG_Bam_R | GGATCCTTTATCATCGTCGTCTTTATAATCAGCGTAAACACCTGACAACG | [11] |
Gene | ||
zil | GTTGACGCTAAATATGCTAAAGAGGAACAACGTGCTTGGAGAGAAATTCACTTATTACCTAATCTTACAATCGAACAAATGGCAGCATTCATTTGGAAATTGTTAGATGATCCATCACAATCTTCAGAGTTGTTATCAGAGGCTAAAAAACTTAATGATAGTCAAGCCCCAAAA | This study |