Fig. 7
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
ZIL-displaying L. lactis removes IL-6 secreted by differentiated THP-1 and differentiated U-937 cells in proportion to the concentration of bacterial cells. ELISA-determined concentrations of IL-6 that remained in the supernatants of LPS-induced differentiated THP-1 cells (a) and differentiated U-937 cells (b) following incubation with ZIL-displaying L. lactis (ZIL). Cont., L. lactis control cells containing empty plasmid pNZ8148. Untr., untreated supernatants. Dose response curves for calculating the concentration of ZIL-displaying L. lactis cells necessary for removal of a 50% of IL-6 from the cell culture supernatants (EC50, half-maximal effective concentration) was determined by curve fitting using four parameters logistic (4 PL) regression model in GraphPad Prism (c). The results are expressed as mean ± standard deviation (SD) of experiments performed in triplicate. **, P ≤ 0.007; ***, P < 0.001 (unpaired t-test)