Fig. 4
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
ZIL-displaying L. lactis removes various amounts of recombinant IL-6 from the solution in a concentration-dependent manner. ELISA-determined concentrations of recombinant IL-6 that remained in the solution following incubation with ZIL-displaying L. lactis (3 × 106–6 × 109 CFU/mL) across four concentrations of recombinant IL-6 (150, 300, 600 and 1200 pg/mL) that were spiked into the PBS buffer (left). ZIL., L. lactis cells containing pSD-ZIL plasmid. Cont., L. lactis control cells containing empty plasmid pNZ8148. Dose response curves for calculating the concentration of ZIL-displaying bacterial cells necessary to remove a 50% of recombinant IL-6 from the solution (half-maximal effective concentration, EC50) determined by curve fitting with four parameters logistic (4 PL) regression model in GraphPad Prism (right). The results are expressed as mean ± standard deviation (SD) of three individual measurements. **, P ≤ 0.006; ***, P < 0.001 (unpaired t-test)