Fig. 3
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
Surface-displayed IL-6-binding affibody ZIL is functional. Representative confocal immunofluorescence microscopy images (a) and flow cytometric analysis (b) showing binding of ZIL-flag-displaying L. lactis to human biotin-conjugated IL-6. ZIL-flag., L. lactis cells containing pSD-ZIL-flag plasmid. Cont., L. lactis control cells containing empty plasmid pNZ8148. Control or ZIL-flag-displaying L. lactis cells were incubated with IL-6-biotin and detected with an anti-biotin antibody, followed by a secondary antibody conjugated to Alexa Fluor 488. The results are presented as mean ± standard deviation (SD) of three individual measurements. ***, P < 0.001 (unpaired t-test). a: Bright-field images (left) and the corresponding fluorescence images (right). Bar scale 20 µm. b: MFI, mean fluorescence intensity