Fig. 2
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
IL-6-binding affibody ZIL is displayed on L. lactis surface. a Representative confocal immunofluorescence microscopy images visualizing ZIL-flag at the bacterial surface and b flow cytometry analysis showing a large increase in mean fluorescence intensity and a shift of the population of ZIL-flag-expressing L. lactis compared to control bacterial cells. ZIL-flag, L. lactis harbouring pSD-ZIL-flag plasmid. Cont., L. lactis harbouring empty plasmid pNZ8148. Engineered bacteria were incubated with an anti-flag antibody and then probed with Alexa Fluor 488 or Alexa Fluor 555-conjugated secondary antibody. The results are expressed as mean ± standard deviation (SD) of three individual measurements. ***, P < 0.001 (unpaired t-test). a: Bright-field images (left) and the corresponding fluorescence images (right). Bar scale 20 µm. b: MFI, mean fluorescence intensity