Fig. 1
From: Targeting IL-6 by engineered Lactococcus lactis via surface-displayed affibody
IL-6 binding affibody ZIL is expressed in L. lactis. a Gene constructs for expression of IL-6 binding affibody ZIL on the surface of L. lactis. USP, gene encoding Usp45 secretion signal (84 bp). ZIL, gene encoding IL-6-binding affibody (174 bp). Flag, epitope tag sequence. AcmA, gene encoding C-terminal domain of AcmA anchoring protein (642 bp). The arrow represents the nisin-inducible promoter. b Coomassie brilliant blue-stained SDS-PAGE gel (left) and Western blot analysis (right) of whole lysates of L. lactis harbouring plasmids pSD-ZIL or pSD-ZIL-flag. Cont., L. lactis containing empty plasmid pNZ8148. Bands representing untagged or flag-tagged ZIL fusion protein are indicated with arrows