Fig. 9

Comparison of process kinetics from optimized cultivations performed in shake flaks RAMOS (200 mL) and stirred tank DASGIP (1.5 L). Legends marked with D illustrate process values from the DASGIP cultivation and R from RAMOS cultivation. a Overlay of OTR-CTR and product formation over the cultivation time of the DASGIP cultivation with highest product titers and a RAMOS cultivation. b Total amount of glucose and acetate consumed over the cultivation time calculated from the difference between initial concentrations and the measured concentration in the daily samples. Dashed curves illustrate the data from RAMOS cultivations and solid curves illustrate the DASGIP cultivations. Error bars indicate one standard deviation (1SD) of the mean with n = 4 for RAMOS and n = 3 for DASGIP cultivations. c, d Illustrate a comparison of averages of the integrated OTR and CTR values (OT-CT) (RAMOS n = 4 and DASGIP n = 3). The blue circle highlights the time of the first acetate feed (96 h)