Fig. 10

Process kinetics of a 10 L stirred tank cultivation where pH was shifted from pH 4.5 to pH 6.5 at 85 h. The culture was prepared with O. nidiformis cultivated in G13.5/C7 medium at 23 °C, 0.3 vvm (180 sL h⁻¹) aeration and dissolved oxygen (DO) was maintained at 5% by increasing the agitation speed. Acetate was fed at 96 h (8 g L⁻¹), 120 h (4 g L⁻¹) and 144 h (4 g L⁻¹) indicated with black triangles. A feed of glucose was performed at 120 h (6 g L⁻¹) indicated with a red triangle. The plots illustrate two sets of data with the full kinetics of the experiment. All curves illustrating the course of the different process parameters are colored according to the colors of the axis labels. Illudin M titers were derived from cell free culture supernatant