Fig. 1

Process kinetics from cultivations in standard shake flasks and RAMOS flasks for production of illudin M with O. nidiformis. Experiments in conventional Erlenmeyer flasks: a batch process used as control, b optimized fed-batch process. Experiments in RAMOS flasks: c batch process used as control, d optimized fed-batch process. The fed-batch cultivations were fed with acetate (8 g L⁻¹) at 96 h indicated with a black triangle and glucose (6 g L⁻¹) at 120 h indicated with a red triangle. All curves illustrating the course of different process parameters are colored according to the color of the axis labels. The highest measured concentration of illudin M in the batch cultivations was ~ 420 mg L⁻¹ at 144 h and in the fed-batch ~ 1000 mg L⁻¹ at 192 h. Illudin M titers were derived from cell free culture supernatant