Bioprocess development as a sustainable platform for eco-friendly alkaline phosphatase production: an approach towards crab shells waste management

Table 5 Kinetic parameters of cell growth and ALP production by B. licheniformis strain ALP3 as affected by different cultivation strategies

Parameters	Shake flask cultivation	Uncontrollable pH Batch Cultivation	Controllable pH Batch Cultivation
Growth parameters
\({ X}_{max}\) (g. L⁻¹)	1.880	2.625	2.52
µ (h⁻¹)	0.131	0.343	0.381
\({ \mu }_{max}\) (h⁻¹)	0.403	0.695	0.489
\(\frac{ dX}{dt}\) (g. L⁻¹. h⁻¹)	0.112	0.238	0.250
Production parameters
\({ P}_{max}\)(IU L⁻¹)	178.56	212.29	186.41
\({P}_{max. specific}\) (IU g⁻¹)	28.034	31.165	28.533
\({ P}_{max. time}\) (h)	30	22	28
\({ Q}_{p}\) (IU L⁻¹ h⁻¹)	5.961	10.824	7.330
-\({Q}_{s}\) (g L⁻¹. h⁻¹)	0.013	0.018	0.016
Yield coefficient parameters
\({ Y}_\frac{p}{s}\) (IU g⁻¹)	182.7	795.4	688.9
\({ Y}_\frac{p}{x}\) (IU g⁻¹)	49.8	39.3	40.7
\({ Y}_\frac{x}{s}\) (g g⁻¹)	1.5	15.8	15.3
Overall cultivation time (h)	32	32	32

X_max, maximal cell dry weight; \(\frac{dX}{dt}\), cell growth rate; µ, specific growth rate; P_max, maximal ALP production; P_{max specific}, specific productivity; \({Q}_{p}\), ALP production rate; \({Q}_{s}\), substrate consumption rate; \({Y}_\frac{p}{x}\), U g⁻¹ of ALP produced per g biomass; \({Y}_\frac{p}{s}\), U g⁻¹ of ALP produced per g substrate consumed; \({Y}_\frac{x}{s}\), g g⁻¹, of biomass produced per g substrate consumed

ISSN: 1475-2859