Bioprocess development as a sustainable platform for eco-friendly alkaline phosphatase production: an approach towards crab shells waste management

Table 4 Analysis of variance for the response surface of ALP production by B. licheniformis strain ALP3 obtained by SCCD

Term	Coefficient estimate	Mean square	t-Stat	F-value	p-value	Confidence level (%)	Contribution (%)
Intercept	121.46	1144.29	166.96	180.17	1.3276E⁻²³
X₁	− 2.38	158.84	− 5.001	25.01	2.32312E⁻⁰⁵	99.997	4.798133
X₂	− 1.83	93.65	− 3.840	14.75	0.0006	99.940	3.68429
X₃	5.01	702.59	10.51	110.63	1.39353E⁻¹¹	100	10.0912
X₁*X₂	4.58	335.67	7.269	52.85	4.28744E⁻⁰⁸	100	9.22715
X₁*X₃	17.99	5180.66	28.56	815.71	2.60377E⁻²³	100	36.2495
X₂*X₃	0.7634	9.32	1.211	1.47	0.2351	76.490	1.53786
X₁*X₁	− 10.70	3608.10	− 23.83	568.11	4.73558E⁻²¹	100	21.5602
X₂*X₂	− 3.60	408.63	− 8.021	64.34	5.93074E⁻⁰⁹	100	7.2557
X₃*X₃	− 2.78	243.05	− 6.186	38.27	8.29518E⁻⁰⁷	96.999	5.5958

	df	SS	MS	F-value	Significance F
Regression	9	10,298	1144	180.17	1.3276E⁻²³	Std. Dev	2.52
Residual	30	190.53	6.351			Mean	109.51
Total	39	10,489				C.V. %	2.30
Multiple R	0.99					PRESS	410.68
R²	0.981					Adeq. Precision	40.87
Adj. R²	0.976					Adeq. Precision	40.87
Pred. R²	0.960

“Std. Dev. is the standard deviation, the coefficient of determination (R²), Adj R² is the adjusted-R², and PRESS is the prediction error sum of squares, C.V is the coefficient of variation

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