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Table 3 Matrix designed for B. licheniformis strain ALP3 SCCD

From: Bioprocess development as a sustainable platform for eco-friendly alkaline phosphatase production: an approach towards crab shells waste management

StdOrder

Trials

 

Variables

ALP productivity (U L−1 min−1)

Type

X1

X2

X3

Actual value

Predicted value

Residual

27

1

Axial

0

0

1.73

117.78

121.806

− 4.0267

9

2

Factorial

− 1

− 1

1

100.62

99.4245

1.1975

12

3

Factorial

1

− 1

1

123.88

121.488

2.3985

15

4

Factorial

1

1

1

126.21

128.518

− 2.3046

34

5

Center

0

0

0

121.85

121.463

0.3877

21

6

Axial

0

− 1.73

0

113.12

113.826

− 0.6992

11

7

Factorial

1

− 1

1

122.72

121.488

1.2353

24

8

Axial

0

1.73

0

107.89

107.491

0.4014

30

9

Center

0

0

0

120.68

121.463

− 0.7755

37

10

Center

0

0

0

121.85

121.463

0.3877

14

11

Factorial

− 1

1

1

92.479

88.1329

4.3463

25

12

Axial

0

0

− 1.73

106.72

104.454

2.2748

16

13

Factorial

1

1

1

127.66

128.518

− 0.8505

33

14

Center

0

0

0

121.85

121.463

0.3877

2

15

Factorial

− 1

− 1

− 1

130.57

126.921

3.6548

18

16

Axial

− 1.73

0

0

88.989

93.4818

− 4.4923

4

17

Factorial

1

− 1

− 1

72.994

77.0086

− 4.0139

38

18

Center

0

0

0

121.85

121.463

0.3877

7

19

2

Factorial

1

1

 − 1

79.683

80.9847

− 1.3013

20

20

Axial

1.73

0

0

87.535

85.2310

2.3043

36

21

Center

0

0

0

120.68

121.463

− 0.7755

40

22

Center

0

0

0

121.85

121.463

0.3877

29

23

Center

0

0

0

121.27

121.463

− 0.1938

31

24

Center

0

0

0

120.68

121.463

− 0.7755

6

25

Factorial

− 1

1

− 1

111.96

112.576

− 0.6121

22

26

Axial

0

− 1.73

0

112.54

113.826

− 1.2808

39

27

Center

0

0

0

121.85

121.463

0.3877

17

28

Axial

− 1.73

0

0

90.152

93.4818

− 3.3290

23

29

Axial

0

1.73

0

107.02

107.491

− 0.4709

19

30

Axial

1.73

0

0

88.698

85.2310

3.4675

5

31

Factorial

− 1

1

− 1

111.09

112.576

− 1.4846

28

32

Axial

0

0

1.73

119.23

121.806

− 2.5726

1

33

Factorial

− 1

− 1

− 1

127.95

126.921

1.0374

32

34

Center

0

0

0

121.27

121.463

− 0.1938

8

35

Factorial

1

1

− 1

80.265

80.9847

− 0.7197

3

36

Factorial

1

− 1

− 1

73.867

77.0086

− 3.1415

26

37

Axial

0

0

− 1.73

106.72

104.454

2.2748

35

38

Center

0

0

0

121.85

121.463

0.3877

10

39

Factorial

− 1

− 1

1

101.78

99.4245

2.3607

13

40

Factorial

− 1

1

1

92.479

88.1329

4.3463

Variables

Code

Coded and actual levels

− 1.73

− 1

0

1

1.73

Crab shells powder

X1

12

24

36

48

60

ZnSO4.7H2O

X2

0.001

0.002

0.003

0.004

0.005

CoCl2.6H2O

X3

0.001

0.002

0.003

0.004

0.005