Table 1 Randomized Plackett–Burman experimental design for evaluating factors influencing ALP production by B. licheniformis strain ALP3
Trails | Variables | ALP productivity (U L−1 min−1) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | Actual value | Predicted value | Residual | |
1 | 1 | –1 | 1 | –1 | –1 | –1 | –1 | 1 | 1 | − 1 | − 1 | 1 | − 1 | 36.7881 | 35.95372 | 0.834416 |
2 | –1 | 1 | –1 | 1 | –1 | 1 | –1 | –1 | 1 | 1 | − 1 | − 1 | 1 | 34.1707 | 38.6583 | − 4.4875 |
3 | –1 | –1 | –1 | 1 | 1 | –1 | –1 | 1 | –1 | − 1 | 1 | 1 | 1 | 72.7038 | 75.73725 | − 3.03343 |
4 | –1 | 1 | –1 | –1 | –1 | –1 | 1 | 1 | –1 | − 1 | 1 | − 1 | − 1 | 33.5891 | 37.43687 | − 3.84771 |
5 | 1 | –1 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | − 1 | 1 | − 1 | 27.9182 | 29.23588 | − 1.31762 |
6 | 1 | 1 | –1 | 1 | –1 | 1 | –1 | –1 | –1 | − 1 | 1 | 1 | − 1 | 36.3519 | 33.83077 | 2.521145 |
7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
10 | –1 | 1 | 1 | –1 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | − 1 | 31.9896 | 33.56903 | − 1.57935 |
11 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 47.2574 | 52.55927 | − 5.30179 |
12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
14 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | –1 | 1 | − 1 | 1 | − 1 | 52.4921 | 50.55264 | 1.939514 |
15 | –1 | –1 | –1 | –1 | 1 | 1 | –1 | –1 | 1 | − 1 | − 1 | 1 | 1 | 65.4334 | 65.93677 | − 0.50333 |
16 | –1 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | − 1 | 1 | − 1 | 1 | 41.7319 | 41.45013 | 0.281867 |
17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 49.0023 | 48.19704 | 0.805335 |
18 | 1 | –1 | 1 | –1 | 1 | –1 | –1 | –1 | − 1 | 1 | 1 | − 1 | − 1 | 61.6528 | 65.84953 | − 4.19669 |
19 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | –1 | 1 | − 1 | 1 | − 1 | − 1 | 58.7446 | 58.69547 | 0.049215 |
20 | 1 | –1 | –1 | –1 | –1 | 1 | 1 | –1 | –1 | 1 | − 1 | − 1 | 1 | 31.5534 | 32.34761 | − 0.79415 |
21 | 1 | 1 | 1 | –1 | 1 | –1 | 1 | –1 | –1 | − 1 | − 1 | 1 | 1 | 52.3467 | 53.95518 | − 1.60843 |
22 | –1 | –1 | 1 | 1 | 1 | 1 | –1 | 1 | –1 | 1 | − 1 | − 1 | − 1 | 63.6885 | 63.0577 | 0.630845 |
23 | 1 | 1 | 1 | 1 | –1 | 1 | –1 | 1 | –1 | − 1 | − 1 | − 1 | 1 | 34.6070 | 34.26699 | 0.34003 |
24 | 1 | 1 | –1 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | − 1 | 1 | 70.8135 | 63.75566 | 7.057863 |
25 | –1 | –1 | 1 | 1 | –1 | –1 | 1 | –1 | –1 | 1 | 1 | 1 | 1 | 47.6937 | 42.06084 | 5.632868 |
26 | –1 | 1 | 1 | 1 | 1 | –1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | − 1 | 57.5814 | 55.0312 | 2.550226 |
Variables | Code | − 1 | 0 | + 1 |
|---|---|---|---|---|
Molasses | X1 | 0.1 | 0.3 | 0.5 |
Arabic gum | X2 | 0.1 | 0.3 | 0.5 |
(NH4)2SO4 | X3 | 0.02 | 0.11 | 0.2 |
NaNO3 | X4 | 0.1 | 0.3 | 0.5 |
Crab shells powder | X5 | 2.5 | 13.75 | 25 |
NaCl | X6 | 0.1 | 0.3 | 0.5 |
MgCl2.6H2O | X7 | 0.02 | 0.06 | 0.1 |
K2HPO4 | X8 | 0.02 | 0.06 | 0.1 |
NaH2PO4 | X9 | 0.02 | 0.06 | 0.1 |
CuSO4.5H2O | X10 | 0.0005 | 0.0015 | 0.0025 |
ZnSO4.7H2O | X11 | 0.0005 | 0.0015 | 0.0025 |
NiSO4 | X12 | 0.0005 | 0.0015 | 0.0025 |
CoCl2.6H2O | X13 | 0.0005 | 0.0015 | 0.0025 |