Fig. 2
Determination of the relative transcriptional level of pgdS gene at different fermentation time. The threshold cycles (CT) for each PCR with different concentrations of cDNA were determined and were compared with that for a standard DNA (the 16S rRNA gene) that was also analyzed at the same time. The y-axis indicates the transcription ratio of pgdS at 12 h, 24 h, 36 h to 6 h. Error bars represent SD calculated from four independent determinations