Table 2 Escherichia coli and Y. lipolytica strains and plasmids used in this study
From: Enhancing very long chain fatty acids production in Yarrowia lipolytica
Strain (host strain) | Plasmid, genotype | References |
|---|---|---|
E. coli strains | ||
EC11 (DH5a) | JMP62-pTEF-TaFAE1-LEU2ex | [14] |
EC61 (DH5a) | JMP62-8UAS-pTEF-TaFAE1-LEU2ex | This work |
JME1112 (DH5a) | JMP62-pTEF-YlDGA1-URA3ex | [21] |
JME4305 (DH5a) | JMP62-URA3ex-8UAS-pTEF-FAR4 | [41] |
Y. lipolytica strains | ||
JMY1882 | MATA ura3-302 leu2-270 xpr2-322 Δdga1Δlro1Δare1Δdga2 pTEF-YlLRO1-URA3ex | [21] |
JMY1884 | MATA ura3-302 leu2-270 xpr2-322 Δdga1Δlro1Δare1Δdga2 pTEF-YlDGA2-URA3ex | [21] |
JMY1892 | MATA ura3-302 leu2-270 xpr2-322 Δdga1Δlro1Δare1Δdga2 pTEF-YlDGA1-URA3ex | [21] |
JMY1915 | MATA ura3-302 leu2-270 xpr2-322 Δdga1Δlro1Δare1Δdga2Δmfe | [34] |
YL12 | JMY1882 pTEF-TaFAE1-LEU2ex | This work |
YL13 | JMY1884 pTEF-TaFAE1-LEU2ex | This work |
YL15 | JMY1892 pTEF-TaFAE1-LEU2ex | This work |
YL51 | JMY1915 pTEF-YlDGA1-URA3ex | This work |
YL53 | JMY1915 pTEF-YlDGA1-URA3ex 8UAS-pTEF-TaFAE1-LEU2ex | This work |
YL62 | JMY1892 8UAS-pTEF-TaFAE1-LEU2ex | This work |