Efficient and scalable synthesis of 1,5-diamino-2-hydroxy-pentane from l-lysine via cascade catalysis using engineered Escherichia coli

Table 1 Comparison the one- and two-stage syntheses of 2-OH-PDA by engineered E. coli

Strategy	B1 (g/L DCW)	B15 (g/L DCW)	l-lysine (g/L)	Hydroxylysine (g/L)	2-OH-PDA (g/L)
One-stage	5	0	19.8 ± 0.3	12.1 ± 0.6	0
	5	0.5	0	0	0.8 ± 0.1
	5	2.5	0	0	0.1 ± 0.04
	5	5	0	0	0
Two-stage	5	5	0	0	9.3 ± 0.7

B1 (pRSFDuet1-K3H, expressing hydroxylase) and B15 (pRSFDuet1-CadA, expressing decarboxylase) were simultaneously added at different concentrations to the reaction mixture simultaneously for the one-stage synthesis. In the two-stage process, B1 hydroxylated l-lysine to produce (2S,3S)-3-hydroxylysine for 24 h, and then B15 was added to decarboxylate hydroxylysine to produce 2-OH-PDA over the next 24 h

ISSN: 1475-2859