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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Efficient and scalable synthesis of 1,5-diamino-2-hydroxy-pentane from l-lysine via cascade catalysis using engineered Escherichia coli

Fig. 3

Effects of decarboxylase (a), l-lysine concentration (b), pH (c), PLP concentration (d), and temperature e on 2-OH-PDA titre. The 2-OH-PDA synthesis reaction contained hydroxylysine (10, 30, 50, and 70 g/L), PLP (0, 0.05, 0.1, and 0.2 mM), and 5 g DCW/L and was incubated at different pH values (4.0, 5.0, 6.0, 7.0, 8.0, and 9.0) and temperatures (20, 30, 40, 50, 60, 70, and 80 °C) with shaking at 200 rpm for different lengths of time (0, 6, 12, 24, and 48 h). Samples (0.3 mL) were collected every few hours

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Microbial Cell Factories

ISSN: 1475-2859

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