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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Efficient and scalable synthesis of 1,5-diamino-2-hydroxy-pentane from l-lysine via cascade catalysis using engineered Escherichia coli

Fig. 1

Effect of lysine transporters on hydroxylysine production. The reaction for hydroxylysine synthesis contained 30 g/L l-lysine, 36 g/L α-ketoglutaric acid, 5 mM Vc, 5 mM FeSO4, and 5 g dry cell weight (DCW)/L and was incubated at 30 °C with shaking at 200 rpm for 30 h. Overexpression of lysine hydroxylase: strain B1 (pRSFDuet1-K3H). Coexpression of lysine hydroxylase and a single lysine transporter protein: strains B2 (pRSFDuet1-K3H-lysP), B3 (pRSFDuet1-K3H-YbjE), B4 (pRSFDuet1-K3H-HisQ), B5 (pRSFDuet1-K3H-HisM), B6 (pRSFDuet1-K3H-HisP), B7 (pRSFDuet1-K3H-argO), B8 (pRSFDuet1-K3H-cadB), and B9 (pRSFDuet1-K3H-argT). Coexpression of lysine hydroxylase and combined lysine transporter proteins: strains B10 (pRSFDuet1-K3H-lysP-YbjE), B11 (pRSFDuet1-K3H-lysP-argO), B12 (pRSFDuet1-K3H-CadB-YbjE), B13 (pRSFDuet1-K3H-CadB-argO), and B14 (pRSFDuet1-K3H-CadB-argT)

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Microbial Cell Factories

ISSN: 1475-2859

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