Fig. 4
From: Engineering heterologous enzyme secretion in Yarrowia lipolytica
Removal of pro sequence promotes the secretion of GFP and \(\alpha\)-Amylase. Both the expression of GFP and \(\alpha\)-Amylase was performed under the control of TEF promoter with or without pro sequence in shake flasks. Changes were measured with comparisons to pro sequence including constructs (column 1 and 3). *, P < 0.05; **, P < 0.01; Measurements were reported as the average values \(\pm\) standard deviations from individual triplicates