Fig. 5
From: Flagella disruption in Bacillus subtilis increases amylase production yield
Deletion of flgE disrupts motility and increase JE1 yield. A Genomic content of the WT and ΔflgE strains. Deletion of flgE was done by replacement with an erythromycin resistance gene (ERM) followed by a terminator. B Swimming assay of WT and ΔflgE strains in triplicates. C Relative mRNA levels of flagella genes measured by qRT-PCR in WT (black) and ΔflgE (red) normalized to WT. N = 3, error bars depict standard error of the mean. D Biolector fermentation JE1 activity in WT (black) and ΔflgE (red) normalized to WT. N = 3, error bars depict standard deviation. E as in (C) but je1zyn mRNA levels