Fig. 4
From: Flagella disruption in Bacillus subtilis increases amylase production yield
sgRNAs targeted against flagella genes increase JE1 amylase activity but not je1zyn mRNA level. A Biolector fermentation JE1 amylase activity in strains expressing sgRNA::flgE, sgRNA::fliR or, sgRNA::flhG (two sgRNAs per gene, grey and white bars) or sgRNA::gfp (green bars) normalized to sgRNA::gfp. N = 3, error bars depict standard deviation. B Relative je1 mRNA level measured by qRT-PCR of strains as in (A) but error bars depict standard error of the mean