Fig. 3
From: Flagella disruption in Bacillus subtilis increases amylase production yield
adapted from Mukherjee and Kearns [26] according to gene function. B Swimming assay of strains expressing sgRNA against flagellar genes or gfp. C qRT-PCR of mRNA of flagellar genes (green boxes in A) in strains expressing sgRNA::flgE, sgRNA::fliR or sgRNA::flhG (two sgRNAs per gene, grey and white bars) or sgRNA::gfp (green bars) normalized to sgRNA::gfp. Schematic illustration of fla/che operon according to (A) is shown below plots. N = 3, error bars depict standard error of the mean
Motility is disrupted by CRISPR-dCas9 targeting of the fla/che operon. A The fla/che operon showing sgRNAs against flgE, fliR, and flhG (red boxes) and qRT-PCR amplicons (green boxes). Gene colors