Fig. 1

Expression dynamics of flagella operon during fermentation. A Culture density during fermentation measured at OD₆₅₀ in triplicates. Error bars depict standard deviation. Dashed line marks sampling time points for RNA-seq B Protein yield measured as JE1zyn activity during fermentations in triplicates. Error bars depict standard deviation. C Heatmap of the temporal expression dynamics across the 32 genes of the flagella operon showing the per gene z-scaled mean of DESeq2’s rlog transformed data. 13 genes (marked with stars) had statistically significant changes in expression levels during the fermentation according to a test with DESeq2 (FDR adjusted p ≤ 0.05, 5 comparisons along the time axis and 4 against expression at first time-point, see Additional File 1: Figure S1 and S2). The columns are sorted according to their position within the flagella operon