Fig. 2

The purification of R12 Dps and R1 Dps1 proteins. A DNA binding assays with R12 Dps and R1 Dps1 proteins. Lane 1: pET-22b(+) plasmid; Lane 2: The plasmid combined with R1 Dps1 protein (in deionized water); Lane 3: The plasmid combined with R12 Dps protein (in deionized water); Lane 4: The plasmid combined with R1 Dps1 proteins (crosslinked with 0.1% glutaraldehyde); Lane 5: The plasmid combined with R12 Dps protein (crosslinked with 0.1% glutaraldehyde). B DNA protection experiments with R12 Dps and R1 Dps1 proteins. Lane 1: pET-22b(+) plasmid; Lane 2: The plasmid following exposure to 80 mM H₂O₂; Lane 3: The resulting plasmid following exposure 80 mM H₂O₂ with the protection of the R1 Dps1 protein; Lane 4: The plasmid following exposure 80 mM H₂O₂ with the protection of the R12 Dps protein; C The purification of R1 Dps1 and R12 Dps proteins. Lane 1: The crude extract of pET-22b(+)-R1Dps1 recombinant E. coli. Lane 2: The crude extract of pET-22b(+)-R12Dps recombinant E. coli. Lane 3: The purified R1 Dps1 protein; Lane 4: The purified R12 Dps protein