Fig. 2

Promotion effect on the secretion of S tag. A Schematic diagram of the SHTXTHHly system. POI: Protein of interest; TEV cs: TEV enzyme cleavage site; Hly218: the last 218 amino acids of hemolysin (A). B SDS-PAGE analysis of culture samples (n = 2). W: Whole-cell lysate; S: Supernatant. The native and S tag-fused tag fusion proteins are indicated above the image. The arrows indicate the tag fusion proteins. C Summary of the expression profiles of the tag fusion proteins. The expression levels were estimated by Image J scanning. All SDS-PAGE was conducted under non-reducing conditions