Table 3 Kinetic parameters of L-carnosine synthase (WT) and the mutant G310A
From: Characterization of a new L-carnosine synthase mined from deep-sea sediment metagenome
β-alanine methyl ester hydrochloridea | L-histidineb | |||||
|---|---|---|---|---|---|---|
Km (mM) | kcat (s−1) | kcat/Km | Km (mM) | kcat (s−1) | kcat/Km | |
WT | 1.35 ± 0.20 | 248.67 ± 7.33 | 184.20 | 235.3 ± 76.75 | 1013.33 ± 195.33 | 4.31 |
G310A | 0.81 ± 0.04 | 141.33 ± 1.33 | 174.48 | 7.73 ± 0.29 | 139.33 ± 0.67 | 18.02 |