Table 1 Primers used for site mutations in this study
From: Characterization of a new L-carnosine synthase mined from deep-sea sediment metagenome
primers | Sequence (5′-3′) |
|---|---|
W133D-F | TGGCCGGCTGACgacCACGCTCCGGTTGTT |
W133D-R | CGGAGCGTGgtcGTCAGCCGGCCAACCCTG |
Y142N-F | GTTGTTGCTGAAACCaacGACGGTGGTCTG |
Y142N-R | TCGTTCAGACCACCGTCgttGGTTTCAGC |
Y142K-F | TTGTTGCTGAAACCaaaGACGGTGGTCTG |
Y142K-R | TCGTTCAGACCACCGTCtttGGTTTCAGC |
S86Q-F | AACGCTcagGGTGAAATGACCGGTACCACCTGGC |
S86Q-R | GCCAGGTGGTACCGGTCATTTCACCctgAGCGTT |
D218E-F | GTGCAACTACAACTGGgaaGGTGAACAGGACCTG |
D218E-R | CAGGTCCTGTTCACCttcCCAGTTGTAGTTGCAC |
G310A-F | CTCTTCTGACgctTCTGGTGACATCTTCCTGGC |
G310A-R | GCCAGGAAGATGTCACCAGAagcGTCAGAAGAG |
S86NG310A-F | GAACGCTaacGGTGAAATGACCGGTACCACC |
S86NG310A-R | GGTGGTACCGGTCATTTCACCgttAGCGTTC |
R270GG310A-F | CAAACCGACCggtGACGGTTCTATCATCATC |
R270GG310A-R | GATGATGATAGAACCGTCaccGGTCGGTTTG |