Fig. 4

Chromosomal copy numbers of each locus for exponential cell populations in LB. The quantitative real-time PCR analysis was performed to measure the location-dependent copy number variation in the target regions of strains with multiple or ectopic replication origins. Data are presented as mean ± standard error. Both oriZ and oriZ’ target regions represent the positions located near the newly introduced ectopic sites, lacZ and intergenic region between dadX and cvrA. Green, red, and blue, and purple bars indicate the wild type, O2 lacZ, and O3 lacZ dadX, and O2’ lacZ dadX, respectively. Copy numbers were calculated from triplicated experiments