Fig. 8
Comparing PIbpA-PET hydrolase expression in LB versus M9 minimal medium. A Experimental protocol to test genomically integrated PIbpA-bglx-HiC construct expression in different culture media. B OD600 and esterase activities of cultures in LB medium including P. putida KT2440, TA7-EG, and TA7-BD, either as the non-hydrolase expressing strains (labelled wt) or with the genomically integrated PIbpA-bglx-HiC cassette. C OD600 and esterase activities of P. putida KT2440, TA7-EG, and TA7-BD, either as the wt strains or with the genomically integrated PIbpA-bglx-HiC cassette, in M9 minimal medium with 20 mM citrate (KT2440), 10 mM TA + 10 mM BD (TA7-BD) or 10 mM TA + 10 mM EG (TA7-EG). Data depict one representative experiment with duplicate cultures