Astaxanthin and eicosapentaenoic acid production by S4, a new mutant strain of Nannochloropsis gaditana

Table 3 Biomass, EPA and ketocarotenoids productivity

	Biomass productivity (g DW L⁻¹ day⁻¹)	Ketocarotenoids (mg g DW⁻¹)	Astaxanthin (mg g DW⁻¹)	Ketocarotenoids (mg L⁻¹ day⁻¹)	EPA (mg g DW⁻¹)	EPA (mg L⁻¹ day⁻¹)
100 µmol m⁻² s⁻¹	0.23 ± 0.02^a	3.33 ± 0.35^a	1.78 ± 0.19^a.b	0.77 ± 0.10^a	13.13 ± 1.52^a.b	3.02 ± 0.44^a
100 µmol m⁻² s⁻¹ + 10 gL⁻¹ Glu	0.25 ± 0.05^a.b	3.54 ± 0.33^a	1.80 ± 0.23^a.b	0.89 ± 0.20^a.b	18.48 ± 2.04^a	4.62 ± 1.06^a
500 µmol m⁻² s⁻¹	0.35 ± 0.01^c	3.72 ± 0.12^a	1.89 ± 0.15^a	1.30 ± 0.06^c	12.36 ± 2.61^b	4.33 ± 0.92^a
500 µmol m⁻² s⁻¹ + 10 g L⁻¹Glu	0.39 ± 0.06 ^c	3.61 ± 0.19^a	1.73 ± 0.26^a.b	1.41 ± 0.23^c	13.38 ± 4.70^a.b	5.22 ± 2.00^a
1000 µmol m⁻² s⁻¹	0.26 ± 0.01^a.b	4.05 ± 0.13^a	2.19 ± 0.07^a	1.05 ± 0.05^a.c	16.05 ± 3.32^a.b	4.17 ± 0.88^a
1000 µmol m⁻² s⁻¹ + 10 g L⁻¹Glu	0.32 ± 0.05^b.c	3.97 ± 0.91^a	2.19 ± 0.50^a	1.27 ± 0.35^b.c	12.57 ± 0.96^a.b	4.02 ± 0.70^a
2000 µmol m⁻² s⁻¹	0.31 ± 0.01^a.b.c	2.12 ± 0.05^b	1.10 ± 0.03^c	0.66 ± 0.03^a	11.37 ± 1.14^b	3.53 ± 0.37^a
2000 µmol m⁻² s⁻¹ + 10 g L⁻¹Glu	0.36 ± 0.03^c	2.31 ± 0.04^b	1.25 ± 0.03^b.c	0.83 ± 0.07^a	12.60 ± 2.02^a.b	4.54 ± 0.82^a

Biomass, EPA and ketocarotenoids productivity of S4 cells grown at different irradiances with or without glucose (Glu). Standard deviations are reported (n = 4). Statistical analysis of data was performed using the one-way analysis of variance (ANOVA) followed by a post hoc Tukey’s test. Letters denote statistically significant variations (p < 0.05)

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