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Fig. 2 | Microbial Cell Factories

Fig. 2

From: PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea

Fig. 2

Analysis of the PhoP- and GlnR-binding region for the SACE_3900 promoter. A 5-biotin labeled SACE_3900-1/-2/-3 DNA fragments were reaction with His6-tagged GlnR and were analyzed using EMSA. B 5-biotin labeled SACE_3900-1/-2/-3 DNA fragments were reaction with His6-tagged PhoP and analyzed using EMSA. C Speculated binding motif of GlnR and PhoP protein in the SACE_3900 upstream promoter. His6-tagged PhoP (0, 0.3 and 0.6 μM) and GlnR (0, 0.4 and 0.8 μM) were added in reaction system. Arrowheads show the free probes without bound protein

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Microbial Cell Factories

ISSN: 1475-2859

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