Table 1 Yeast strains, plasmids, and genes used in this study

CEN.PK111-9A

H3892

S. cerevisiae (MATa his3-Δ1 URA3 leu2-3,112 TRP1 MAL2-8c SUC2)

^a

CEN.PK113-7D

H3887

S. cerevisiae (MATa HIS3 URA3 LEU2TRP1 MAL2-8c SUC2)

^a

PHB production strains

PHB_glu

H5696

H3887 with integration of phaA, phaB1, and phaC1 genes into X-3 EasyClone locus (plasmid B11787)

This article

PHB_cbp

H5716

H3892 with integration of phaA, phaB1, and phaC1 genes into X-3 EasyClone locus and ura3Δ::cbp (from plasmid B9403), CDT-1 (plasmid B8444)

This article

PHB_GH1-1

H5717

H3892 with integration of phaA, phaB1, and phaC1 genes into X-3 EasyClone locus, ura3Δ::GH1-1 (from plasmid pSS20), CDT-1 (plasmid B8444)

This article

cbp_control

H5718

H3892 ura3Δ::cbp (from plasmid B9403), CDT-1 (plasmid B8444)

This article

GH1-1_control

H5719

H3892 ura3Δ::GH1-1 (from plasmid pSS20), CDT-1 (plasmid B8444)

This article

B11787

pTEF1-phaA-tENO1-pTDH3-phaB1-tSSA1-pPGK1-phaC1-tCYC

This article

B9403

pPGK1-cbp-1-tENO1, LEU2, 2µ, kanR

This article

pSS20

pADH1-GH1-1-tENO1, LEU2, CEN/ARS, kanR

[28]

B8444

pPGK1-CDT-1-tENO1, URA3, CEN/ARS, kanR

[28]

oJR004

CCGACATAAGCTGGACCAGTA

This article

oJR025

TTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCGGAAAGAGTGAGGAACTATCGCATA

This article

oJR026

TAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTCTGCCTATTTAACGCCAACGTTG

This article

oJR064

ACGAAGGAAGGAGCACAGAC

This article

oJR065

GACCGAGATTCCCGGGTAAT

This article

Acetyl-CoA acetyltransferase

phaA

Cupriavidus necator, GenBank KP681582

[30]

Acetoacetyl-CoA reductase

phaB1

C. necator, GenBank KP681583

[30]

PHA synthase

phaC1

C. necator, GenBank KP681584

[30]

Cellobiose phosphorylase

cbp

Ruminococcus flavefaciens FD-1, GenBank NZ_ACOK01000116.1

[31]

β-glucosidase

GH1-1

Neurospora crassa, NCU00130, GenBank 3872338

[13]

Cellobiose transporter

CDT-1

N. crassa, NCU00801, GenBank 3879950

[13]