Fig. 7
From: Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a
Phenotypic characterization of atp mutants. A Growth curves of atp mutants in minimal medium supplemented with glucose. B Normalized expression analysis by qPCR of atpI, atpB and atpE after three hours of growth. The ΔCt expression values were normalized to hctA and CysG and represented as the median percentage of expression of each gene in the WT strain. The statistical analysis shows the results of a two-way ANOVA (panel B) followed by Tukey’s HSD multiple comparison tests. P values: P ≤ 0.001 (***), P ≤ 0.01 (**), P ≤ 0.05 (*), NS not significant