Fig. 5

Effect of the terminator sequences on mutagenesis efficiency. A Average mutagenesis efficiency expressed as the percentage of colonies identified as mutants by colony PCR genotyping for every terminator sequence tested in three independent biological replicates. The colour code (scale bar shown on the right side of panel B represents the termination strength as determined by Chen et al. [11]. The statistical analysis shows the results of a one-way ANOVA followed by a Holm-Sidak’s multiple comparisons test comparing all values to the control sample (deletion oligonucleotide). P values: P ≤ 0.001 (***), P ≤ 0.01 (**), P ≤ 0.05 (*), P ≤ 0.1 (0.1), N.S. (not significant). B Average mutagenesis efficiency represented as colour-coded percentage of colonies identified as mutants by colony PCR genotyping in correlation to the ΔGfree of the secondary structure formation of the terminator sequences as calculated by the Vienna RNA suite [27]. The curved dotted solid lines represent the 95% confidence interval of the linear regression (solid line). P value of R²: P ≤ 0.01 (**)