Table 1 Bacterial strains and plasmids used in this study

E. coli BL21(DE3)

F⁻ ompT hsdS_B(r_B⁻m_B⁻) gal dcm rne131 λ(DE3)

Transgen Biotec

E. coli Trans 5α

F-φ80 lac ZΔM15 Δ (lacZYA-argF) U169 endA1 recA1 hsdR17(rk⁻, mk⁺) supE44λ-thi-1 gyrA96 relA1 phoA

Transgen Biotec

HM-CT

E. coli BL21(DE3) containing pCLES, pHM-CT, and pTrc-lyc

This study

MM-CT

E. coli BL21(DE3) containing pCLES, pMM-CT, and pTrc-lyc

This study

LM-CT

E. coli BL21(DE3) containing pCLES, pLM-CT, and pTrc-lyc

This study

pAC-lyc

pACYDuet-1 derivative carrying genes CrtE, CrtI, CrtB

[34]

pCLES

pET28a ( +) derivative carrying genes mvaE, mvaS, T7 promoter, Kan^R

[35]

pTrc-lyc

pTrchis2B derivative carrying genes CrtE, CrtI, CrtB

This study

pHM-CT

pACYDuet-1 derivative carrying genes MVK, PMK, MVD, IDI, T7 promoter, p15A ori, Cm^R

This study

pMM-CT

Recipient plasmid with Trc promoter, derived from pACYDuet-1, carrying genes MVK, PMK, MVD, IDI, p15A ori, Cm^R

This study

pLM-CT

Recipient plasmid with Trc promoter, derived from pACYDuet-1, carrying genes MVK, PMK, MVD, IDI, pSC101 ori, Cm^R

This study

pHM-IDI

Recipient plasmid with T7 promoter, Golden gate ligation fragment for library construction and IDI ORF, p15A ori, CmR^R

This study

pMM-IDI

Recipient plasmid with Trc promoter, derived from pACYDuet-1, Golden gate ligation fragment for library construction and IDI ORF, p15A ori, CmR^R

This study

pLM-IDI

Recipient plasmid with Trc promoter, derived from pACYDuet-1, Golden gate ligation fragment for library construction and IDI ORF, pSC101 ori, CmR^R

This study

pEA-MVK

Donor plasmid with MVK ORF and Bsa I sites, KanR^R

This study

pEA-PMK

Donor plasmid with PMK ORF and Bsa I sites, KanR^R

This study

pEA-MVD

Donor plasmid with MVD ORF and Bsa I sites, KanR^R

This study