Fig. 6From: Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolismHigh-cell-density fermentation with mutant JQ08. The fermentations were performed in a 10Â L bioreactor. Strain JQ08 was initial cultivated in the inorganic salt medium containing glycerol as the sole carbon source. When WCW reached about 0.2Â g/mL, glucose was added into the medium for inositol production. Glucose was supplemented in batches. The DO was maintained at about 30% when glycerol was used as the carbon source for efficient cell growth. Then the level of DO was changed to 30% A, 15% B or 5% C after the addition of glucose for inositol biosynthesisBack to article page