Table 1: Non-natural platform chemicals and strategies used for microbial rewiring
From: Rebooting life: engineering non-natural nucleic acids, proteins and metabolites in microorganisms
Target product | Host strain | Metabolic rewiring strategies | Fermentation conditions | Titer (g/L) | References | ||
|---|---|---|---|---|---|---|---|
Culture | pH | Temp (oC) | |||||
1,3-BDO | E. coli | Addition of ADH, Nox, DERA, AKR and FDH | Batch | 7.4 | 29 | 7.7 | [105] |
E. coli | Overexpression of DERA genes | Batch (500 mL bioreactor) | 7.0 | 37 | 1.1 | [106] | |
C. necator | Overexpression of phaA and phaB1; expression of bld, dra; deletion of sucCD and phaC1 | Batch | 6.9 | 30 | 2.97 | [21] | |
1,4-BDO | E. coli | Overexpression of ribose 5-phosphate (R5P)-dependent PLP pathway from B. subtilis involving two enzymes i.e., PdxS and PdxT | Batch | 7.0 | 37 | 1.41 | [14] |
1,5-PeDO | E. coli | Enhancement of downstream lysine pathway, desensitize lyine mediated feedback inhibition and deletion of iclR | Batch (Shake- flask) | 7.0 | 37 | 0.97 | [14] |
E. coli | Artificial metabolic modules were used to successively convert lysine into 5-hydroxyvalerate and 1,5-PeDO, overexpression of pntAB | Batch (Shake- flask) | 7.0 | 37 | 0.39 | [27] | |
4-Methyl-pentanol | E. coli | Design of high yielding pathway with enzymes selected from nine different organisms | Batch (Culture tube) | - | 30 | 0.193 | [43] |
2‑Methyl‑1‑butanol | C. crenatum | Construction of metabolic pathway using ILV2, ILV3, ILV5, kivd and adh2 genes; fermentation conditions optimization of pH, temperature, incubation time and IPTG concentration | Batch | 6.5 | 32 | 4.87 | [19] |
Malonic acid | E. coli | Introduction of β-alanine pyruvate transaminase (PA0132) from P. aeruginosa and semialdehyde dehydrogenase; deletion of the ydfG gene | Fed-batch | 7.0 | 37 | 3.60 | [35] |
6-aminocaproic acid | E. coli | Overexpression of L-lysine α-oxidase, series of chain elongation cycles and an aldehyde dehydrogenase | Batch | NA | 37 | 0.046 | [33] |