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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Bottom-up synthetic biology approach for improving the efficiency of menaquinone-7 synthesis in Bacillus subtilis

Fig. 5

Effects of improved cofactor regeneration on MK-7 synthesis. a Schematic diagram depicting the construction of stains with an improved NADPH supply. b MK-7 production in BS008, BS010, and BS011 after 120 h fermentation. c Growth curves of BS010 and BS011. d and e Intracellular NADH and NADPH levels in wildtype and recombinant strains. f Relative expression of the modified genes in BS011 compared with that of the corresponding genes in the control strain BS168. The transcript levels of dxs, dxr, fni, menF, aroA and menA were regarded as 1 in strain BS168. Results are presented as the mean of three replicates and error bars represent the standard deviation (SD)

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Microbial Cell Factories

ISSN: 1475-2859

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