Table 2 Oligonucleotides used in this study
From: Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
Primers | Sequence (5’–3’ with restriction sites underlined) |
|---|---|
HpaB-NdeI-F | GGGCCTCATATGAAACCAGAAGATTTCCGCGCCAG |
HpaB-SpeI-R | GGGCCCACTAGTTATTTCAGCAGCTTATCCAGCATGTTGA |
HpaC-NdeI- F | GGGTTTCATATGCAATTAGATGAACAACGCCTGCGC |
HpaC-SpeI-R | GGGCCCACTAGTTAAATCGCAGCTTCCATTTCCAGCATCA |
pZM1-HpaC-F | CTAGCGAAAGGAGGAGTTGACAATTAATCATCGGCTCGTATAATGTGTGGA |
pZM1-HpaC-R | AGAGTTTGTAGAAACGCGTCGACTCCTC |
MatB-F-BamHI | CCGGGATCCAAAGGAGGACAACCATGAGCAACCATCTTTTCGACG |
MatB-R | CGACCTCCTTTAGCACCTTACGTCCTGGTATAAAGATCGGC |
MatC-F | CGTAAGGTGCTAAAGGAGGTCGAAGATGGGTATTGAATTACTGTCCATAGG |
MatC-R-MfeI | CCGCAATTGTCAAACCAGCCCGGGC |
pEKEx3-MatBC-F1 | AGCGCATTGTTAGATTTCATACACGG |
pEKEx3-MatBC-F | CAGGAACCGTAAAAAGGCCGCGTTG |
pEKEx3-MatBC-R | TTGTAGAAACGCAAAAAGGCCATCC |
pEKEx3-MatBC-R2 | TGAAATCTAACAATGCGCTCATCGTCA |
pZM1-HpaBC-F | CGGCCTTTTTACGGTTCCTGTTGTAGAAACGCGTCGACTCCTCC |
pZM1-HpaBC-R | GCCTTTTTGCGTTTCTACAATCCCAGTCACGACCCTAGGG |