Table 1 Strains and plasmids used in this study
From: Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum
Plasmids or strains | Relevant properties | Source or references |
|---|---|---|
Escherichia coli DH5α | Cloning of plasmids | Invitrogen |
E. coli BL21 Starâ„¢ (DE3) | Template for cloning of HpaB and HpaC | Invitrogen |
C. glutamicum (DE3) | Host, integrating T7 RNA polymerase | [43] |
pZM1 | An E. coli-C. glutamicum shuttle vector, pHM1519 ori, KanR | [30] |
pEKEx3 | An E. coli-C. glutamicum shuttle vector, pBL1 ori, SpecR | [32] |
pETM6-RgTAL | pETM6 carrying R. glutinis TAL | [19] |
pZM1-HpaB | pZM1 carrying HpaB from E. coli | This study |
pZM1-HpaC | pZM1 carrying HpaC from E. coli | This study |
pZM1-HpaBC | pZM1 carrying HpaB and HpaC from E. coli | This study |
pZM1-RgTAL-x4CL-yCHS-zCHI | pZM1 carrying R. glutinis TAL, 4CL, CHS, and CHI. X means genes from Arabidopsis thaliana, Petroselinum crispum, and Vitis vinifera; y means genes from Petunia hybrida and Citrus maxima (Cm); z means gene from Cm and Medicago sativa. | This study |
pACYC-MatBC | pACYCDuet-1 carrying R. trifolii MatB and MatC | [24] |
pEKEx3-MatB | pEKEx3 carrying R. trifolii MatB | This study |
pEKEx3-MatBC | pEKEx3 carrying R. trifolii MatB and MatC | This study |
pEKEx3-MatBC-HpaBC | pEKEx3 carrying R. trifolii MatB and MatC, and E. coli HpaB and HpaC | This study |