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Fig. 1 | Microbial Cell Factories

Fig. 1

From: SynBioStrainFinder: A microbial strain database of manually curated CRISPR/Cas genetic manipulation system information for biomanufacturing

Fig. 1

Statistical summary of the SynBioStrainFinder database. a Main data in SynBioStrainFinder, including species number (SN), species with culture medium (SCM), sequenced species (SS), species with reported compounds (SC), and species with CRISPR/Cas systems (SCS). b Proportions of publications reporting four gene-editing methods (zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), Cre/loxp, and CRISPR/Cas) annually. c CRISPR/Cas system construction and delivery type. d Frequently used promoters for Cas9 and sgRNA expression. *, corresponding promoter and its deformation. e Homologous arm length of donor DNA for HDR. f Commonly used sgRNA design tools. g CRISPR/Cas system gene-editing type. h Top 10 species with high strain-related compound counts

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Microbial Cell Factories

ISSN: 1475-2859

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