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Fig.6 | Microbial Cell Factories

Fig.6

From: Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota

Fig.6

C. butyricum-derived EVs faecal transplantation improved UC by improving M1/M2 balance. a Donor mice were randomly divided into 4 groups: DSS−EVs−, DSS−EVs+ , DSS+ EVs−, and DSS+ EVs+ . Fecal sample from donor mice was transplanted to DSS-induced colitic mice. b DAI, c body weight change, and d colon length was measured. e Representative photomicrographs of H&E and AB-PAS stained of colon tissues. Scar bars were 100 μm. f Colonic mucosa was analyzed by Western blotting to detect the expression of M2 macrophage markers (Arg1 and IL-10) and M1 macrophage markers (NOS2 and TNF-α). g Flow cytometry staining of LPMCs for CD16/32 (M1 macrophage marker) and CD206 (M2 macrophage marker). The ratio of M1 and M2 macrophage was shown at the right panel. h Representative immunofluorescence staining of F4/80 (macrophage markers) and CD206 (M2 macrophage markers). Scar bars were 100 μm. Data are presented as means ± SD, n = 6 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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