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Fig.5 | Microbial Cell Factories

Fig.5

From: Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota

Fig.5

Antibiotics application impaired the promotive effect of C. butyricum-derived EVs on M1/M2 macrophage balance. a Mice were treated with antibiotics to deplete the gut microbiota for 14 consecutive days, followed by oral administration of DSS in the presence or absence of C. butyricum-derived EVs in the next 5 days, and were euthanized 5 days later (n = 6 per group). b body weight change, c DAI, and d colon length were measured at the indicated time points. e Representative H&E, AB-PAS (Scar bars was 100 μm), Arg1, and IL-10 (Scar bars were 50 μm) staining of colon sections. f Expression of M2 macrophage markers (Arg1 and IL-10) and M1 macrophage markers (NOS2 and TNF-α) were measured by Western blotting. g LPMCs were isolated, stained for CD16/32 (M1 macrophage marker) and CD206 (M2 macrophage marker), and determined by flow cytometry. The ratio of M1 and M2 macrophage was shown at the right panel. h Representative immunofluorescence staining of F4/80 (macrophage markers) and CD206 (M2 macrophage markers). Scar bars were 100 μm. Data are presented as means ± SD, n = 6 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.

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Microbial Cell Factories

ISSN: 1475-2859

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