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Fig.2 | Microbial Cell Factories

Fig.2

From: Commensal bacteria-derived extracellular vesicles suppress ulcerative colitis through regulating the macrophages polarization and remodeling the gut microbiota

Fig.2

C. butyricum-derived EVs improved M2 macrophage polarization after DSS challenge. a Intestinal permeability measurement by intragastrical administration of FITC-dextran (600 mg/kg). b Expression of barrier-associated proteins (MUC2 and ZO-1), M2 macrophage markers (Arg1 and IL-10), and M1 macrophage markers (NOS2 and TNF-α) were measured by Western blotting. c Representative immunofluorescence staining of F4/80 (macrophage markers) and CD206 (M2 macrophage markers). Scar bars were 100 μm. d LPMCs were isolated and determined by flow cytometry; CD16/32 and CD206 were used as the marker of M1 and M2 macrophages, respectively. The ratio of M1 and M2 macrophages was shown on the right panel. e Colon tissues were stained by immunohistochemistry for Arg1 and IL-10 as M2 macrophage markers. Scar bars were 50 μm. Data are presented as means ± SD, n = 6 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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