Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR

Table 6 Stain and plasmids used in this study

	Description	Source
Strain
Escherichia coli JM109	recA1, endA1, gyrA96, thi-1, hsdR17 (r_K^– m_K⁺), e14^–(mcrA^–), supE44, relA1, Δ(lac–proAB)/F′ [traD36, proAB⁺, lacI^q, lacZΔM15]	Toyobo
Plasmid
pBSP_RephaC_ARpct	pBluescript KS⁺ derivative containing the engineered chimeric PHA synthase gene phaC_AR and pct from Megasphaera elsdenii under the P_Re promoter from the phb operon in Ralstonia eutropha (Cupriavidus necator)	[33]
pBSP_RephaC_ARpctalkK	pBSP_RephaC_ARpct derivative containing alkK from Pseudomonas putida	[36]

ISSN: 1475-2859