Table 3 Biosynthesis of 2HB-containing ternary copolymers
Monomer supplying genes | Entry | Precursor concentration (g L−1)a | Cell dry weight (g L−1) | Polymer production (g L−1) | Monomer composition (mol%) | Mw (× 105) | Mn (× 105) | Mw/Mn | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2HB | 3HB | 3HP | 4H2MB | 5HV | 6HHx | ||||||||||
pct, alkK | 23 | 2HB 2.5 | 3HP 1.0 | 3HB 2.5 | 3.10 ± 0.03 | 0.26 ± 0.03 | 4.4 | 57.2 | 38.3 | – | – | – | 3.7 | 1.2 | 3.2 |
pct, alkK | 24 | 2HB 2.5 | 4H2MB 1.0 | 3HB 2.5 | 3.53 ± 0.16 | 0.39 ± 0.03 | 26.8 | 71 | – | 2.2 | – | – | 2.3 | 1.0 | 2.4 |
pct, alkK | 25 | 2HB 2.5 | 5HV 1.0 | 3HB 2.5 | 3.15 ± 0.07 | 0.45 ± 0.03 | 31.6 | 65.3 | – | – | 3.1 | – | 1.6 | 0.7 | 2.4 |
pct, alkK | 26 | 2HB 2.5 | 6HHx 1.0 | 3HB 2.5 | 2.98 ± 0.06 | 0.33 ± 0.01 | 25.3 | 73.6 | – | – | – | 1.1 | 1.1 | 0.5 | 1.9 |